Foam Vitrobot dewar set and accessories

NanoSoft's foam vitrification dewar set and accessories are compatible with the Vitrobot, but can  provide a robust and easy option for custom, home-made Cryo-EM sample preparation systems. Furthermore, these parts are interchangeable with FEI parts. The design for the provides an improved interface, when used in conjunction with the igloo to create a better seal on the foam dewar. This combination facilitates boiled, cold nitrogen gas in the head space above the dewar, allowing for safer grid transfer to the grid box.

The grid box ring sits at the same height as the Vitrobot version (so grid boxes sit at same depth into dewar), but is slightly thicker and remains in contact with liquid nitrogen longer if the nitrogen level gets low. The ethane cup dimensions mimic those of the Vitrobot ethane cup, and the spider as well. These parts perform as well as the Vitrobot system. 

The ethane condenser tightly interfaces with Metal Spider as well as the spider from the FEI/Thermo Fisher styrofoam dewar set. The user places the spider normally and inserts the condenser without the need to handle an ethane nozzle and can instead focus solely on the liquid ethane level, using just one hand to control ethane flow rate. The condenser should reduce wasted ethane gas and the time required to fill the cup with liquid ethane.

The Igloo is a nearly enclosed cover that sits atop the Vitrobot dewar, with an opening large enough for comfortable grid button access and grid transfer.  The volume enclosed by the Igloo fills with cold gaseous nitrogen, creating a dry space with improved protection versus the ambient environment. The material is compatible with liquid nitrogen temperatures, allowing for easy refill with the Igloo on the dewar. The top surface of the Igloo remains comfortable to the touch, easy for steady transfer. A tweezer holder is integrated into the top surface of the Igloo providing a slot into which the Vitrobot tweezers can be placed. 

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