PELCO easiGlow cryo-em glow discharge system and accessories

The PELCO easiGlow Glow Discharge Cleaning System is a compact, quick and easy to use standalone system. It is primarily designed for cleaning TEM grids and hydrophilisation of TEM carbon support films, which have the tendency to be hydrophobic. A glow discharge treatment with air will make a carbon film surface negatively charged (hydrophilic) which allows aqueous solutions to spread easily.

Glow discharge treatment of TEM grids removes adsorbed hydrocarbons, cleaning them while making them hydrophilic. The PELCO easiGlow is an affordable and easy to operate glow discharge system for any TEM lab.

The practical chamber size with its newly designed glow discharge head and adjustable stage with glass slide holder allows for easy loading and fast turnaround times. The system is fully microprocessor controlled and includes an intelligent touch screen device for control and display of operation parameters. The PELCO easiGlow supports both hydrophilic and hydrophobic treatment with either negative or positive charge and includes two separately controlled gas inlets. The required vacuum level is set by an electronically controlled precision proportional valve. This novel approach eliminates manual setting with a needle valve. A gentle venting procedure ensures that the TEM grids are not disturbed when the system is vented.

Glow Discharge Methods Supported

Features:

• Optimised for cleaning TEM grids
• Precise and easy vacuum setting
• Short cycle times
• Consistent results
• Auto, programmed and manual mode
• Intuitive touch screen for display and operation
• Supports hydrophilic/hydrophobic and negative/positive modes
• Affordable, compact, standalone system

Technical Data

PELCO easiGlow single chamber system: Available without optional extras

PELCO easiGlow dual chamber system: Ideal for regular use of hydrophilic and hydrophobic surface treatments, a dual glow discharge system is available to avoid cross contamination of the glow discharge chambers. Hydrophobic surfaces are preferable when organic solvents are used

PELCO easiGlow discharge set includes: PELCO easiGlow unit, Vacuum pump with all accessories, Rubber gasket to protect the glass chamber edge and PELCO Grid Holder Block

Manufactured in USA

Specifications:

Optional accessories and replacement parts:

1. Reference for glow discharge and use of amylamine to obtain Hydrophobic; positively charged surfaces: Hayat, M.A.2000. Principles and Techniques of Electron Microscopy Biological Applications, page 237-241. Cambridge University Press. ISBN 052163287

2. Reference for glow discharge and use of subsequent Magnesium Acetate treatment to obtain Hydrophylic; charged surfaces: Portmann, R., and Koller, Th. 1976. The divalent cation method for protein-free spreading of nucleic acid molecules. In Proceedings of the 6th European Congress on Electron Microscopy, Vol.2, p546.